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KMID : 0359319970370030613
Korean Journal of Veterinary Research
1997 Volume.37 No. 3 p.613 ~ p.618
Studies on development of ELISA Kits for T-2 toxin
Yoon Hwa-Joong

Kim Tae-Jong
Lee Sung-Yun
Abstract
Direct competitive enzyme linked immunosorbent assay(ELISA) with monoclonal antibodies have been studied for quantitative determination of T-2 toxin from the mold corn. The T-2HS, T-2HS-BSA, T-2HS-HRP and monoclonal antibodies against T-2 toxin produced in the studies were qualified for quantitative ELISA test of T-2 toxin. The meanrecovery rate from ground com spiked T-2 toxin was 83%. The meaning range of the T-2 test was 60ng to 2§¶
. According to the recovery results with the com spiked T-2, the tests proved to be suitable in the screening of the moldy feed samples for the presence of T-2 toxin and will be able to become the basis of the ELISA test for the quantitative screening kits of T-2 toxin.
KEYWORD
Enzyme Linked Immunosorbent Assay(ELISA) , T-2 toxin , T-2HS-BSA , T-2HS-HRP , Monoclonal Antibody
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